General Cell Based Assays
Meso Scale Discovery R-PLEX Human Renin ASY
The R-PLEX Human Renin Assay from Meso Scale Discovery is a multiplex immunoassay that measures active renin in human serum plasma and cell culture supernatants This assay utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity a broad dynamic range and requires a low sample volume The assay can be fully automated and has a rapid 2 5 hour turnaround time The R-PLEX assay quantitatively measures renin levels and is designed for use in biomarker research drug discovery and development This product requires the MSD QuickPlex SQ120 instrument and provides components for 96 tests in the kit
Enzo Life Sciences ER-ID Red assay kit (GFP-CERTIFIED) (1x1Kit)
Endoplasmic Reticulum staining with minimal toxicity to cells. Applications: Fluorescence microscopy. Contents: ER-ID Red detection reagent: 50μl. Hoechst 33342 nuclear stain: 50μl. 10X assay buffer: 15ml
Abcam Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric).
Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric).
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery RPLEX HUM BclX/Bcl2L1 ASY
The R-PLEX Human Bcl-xL/Bcl2-L-1 Assay Kit from Meso Scale Discovery is used to quantitatively measure Bcl-xL and Bcl2-L-1 protein levels in human samples such as serum plasma and cell lysates The assay utilizes electrochemiluminescence (ECL) detection and provides high sensitivity broad dynamic range and rapid results This multiplex assay allows you to measure both proteins in a single well saving sample volume and time The kit includes a 96-well plate coated with capture antibodies detection antibodies calibrators and buffers needed to run the assay Results are obtained in as little as 3 5 hours
Meso Scale Discovery U-PLEX Human IL-17D ASY
The Meso Scale Discovery U-PLEX Human IL-17D Assay Kit is a multiplex immunoassay for measuring IL-17D in human serum plasma and cell culture supernatant It uses electrochemiluminescence detection offering high sensitivity a wide dynamic range and low sample volume needs The kit includes a 96-well plate with capture antibodies detection antibodies with electrochemiluminescent labels calibrators and diluents It quantifies IL-17D with minimal hands-on time using an MSD instrument providing a simple rapid method to measure IL-17D important for immune regulation
Meso Scale Discovery R-PLEX Rat IL-2 ASY
The R-PLEX Rat IL-2 Assay Kit from Meso Scale Discovery measures rat interleukin 2 (IL-2) in various biological fluids using MSD s electrochemiluminescent detection technology It offers high sensitivity a wide dynamic range and low sample volume requirements The kit includes a 96-well plate with capture antibodies detection antibodies calibrators and necessary reagents Results are available in 3 5 hours using an MSD instrument This assay quantitatively measures rat IL-2 crucial for the immune system
Aat Bioquest Fluo-8, AM | 1mg
Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8 dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ∼490/∼520 nm. Fluo-8 AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading.
Cell Biolabs Inc Radius™ 24-Well Cell Migration Assay Kit, 24 assays
Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. The Radius™ 24-Well Cell Migration Assay Kit uses a proprietary 24-well plate to monitor migration of cells. Each plate well contains a 0.68 mm non-toxic, biocompatible hydrogel spot where cells do not attach. Adherent cells are seeded and attach everywhere but the Radius™ Gel coated area. Once firm attachment is achieved, the hydrogel is quickly removed to expose a cell-free region across which cells can migrate. The kit provides qualitative or quantitive results with real-time or endpoint analysis, and it is compatible with most HCS and liquid handling instruments. Radius Cell Migration Assays are also available as well in 48-well, 96-well and 384-well formats, and Radius 24-Well Assay Kits are available with wells precoated with Collagen I, Fibronectin or Laminin to promote adhesion of certain cell types.
Cell Biolabs Inc OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size, 20 assays
The OxiSelect™ In Vitro ROS/RNS Assay Kit is an assay for measuring the total free radical presence of a sample. The assay employs a proprietary quenched fluorogenic probe, DCFH-DiOxyQ), which is based on similar chemistry to the popular DCFH-DA. The DCFH-DiOxyQ probe is first primed with a quench removal reagent, and subsequently stabilized in the highly reactive DCFH form. In this reactive state, ROS and RNS species can react with DCFH, which is rapidly oxidized to the highly fluorescent 2’, 7’-dichlorodihydrofluorescein (DCF) . The DCFH-DiOxyQ probe can react with hydrogen peroxide, peroxyl radical, nitric oxide, and peroxynitrite anion. These free radical molecules are representative of both ROS and RNS, thus allowing for measurement of the total free radical population within a sample. The kit has a detection sensitivity limit of 40 nM for H2O2. Each trial size kit provides sufficient reagents to perform up to 20 assays, including standard curve and unknown samples.
Cell Biolabs Inc OxiSelect™ Superoxide Dismutase Activity Assay Kit, 100 assays
Reactive oxygen species (ROS), such as superoxide and hydrogen peroxide, are constantly produced during metabolic processes in all living species. Superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen, is one of the most important antioxidative enzymes. SOD enzymes are classified into three groups: cytosolic Cu/Zn-SOD, mitochondrial Mn-SOD, and extracellular Ec-SOD. The OxiSelect™ Superoxide Dismutase Activity Assay is a fast and reliable kit for the measurement of SOD activity from cell lysate, plasma, serum, tissue homogenates. The assay uses a xanthine/xanthine oxidase (XOD) system to generate superoxide anions. The included chromagen produces a water-soluble formazan dye upon reduction by superoxide anions. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, SOD standards and unknown protein samples.
Enzo Life Sciences ER-ID Green assay kit (1x1Kit)
Endoplasmic Reticulum staining with minimal toxicity to cells. Applications: Fluorescence microscopy. Contents: ER-ID Green detection reagent: 50μl. Hoechst 33342 nuclear stain: 50μl. 10X assay buffer: 15ml
Mabtech Inc FluoroSpot Plus: Human IFN-γ/IL-2/TNF-α, Pre-coated 96-well PVDF membrane plates, 10 plates
The MABTECH FluoroSpot Plus kit is designed for sensitive and multiplexed detection of human biomolecules at a single-cell level, combining the advantages of ELISpot with the capability to analyze multiple analytes simultaneously. Suitable for research use, it supports large-scale screenings and features pre-coated plates for easy assays.
- Detects human IFN-γ, IL-2, and TNF-α
- Utilizes FluoroSpot assay method
- Includes pre-coated 96-well PVDF membrane plates
- Provides robust assay performance
- Supports freshly prepared or cryopreserved cells
- Has a shelf life of at least 12 months
- Requires storage at 4-8 °C for reagents and room temperature for plates
- Ships at ambient temperature
- Offers fluorescent detection capabilities
- Includes positive control and co-stimulatory antibodies
Cayman Chemical EstradIol ELISA KIt 480Wel
Cayman’s Estradiol ELISA Kit is a competitive assay that can be used for the quantification of estradiol in plasma and serum. This assay has a range of 0.61-10,000 pg/ml with a sensitivity of approximately 20 pg/ml (80% B/B0)
Cayman Chemical CalcIum asy KIt 192Wel
Cayman's Calcium Assay provides a quick, reliable method for determining total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates. The assay utilizes an optimized variant of the well-established o-Cresolphthalein-calcium reaction in which a vivid purple complex that absorbs between 560 and 590 nm is formed in the presence of calcium.